Liquid Handling with the 12-Pette
®
Multichannel Pipettor
Liquid is drawn into the tips when the dispensing trigger is
released.
Liquid is dispensed from the tips when the trigger is pulled.
Residual liquid is “blown out” from the tips when the trigger
is pulled beyond the first stop.
Always pull and release the trigger gently, smoothly, and
consistently.
Make it a habit to quickly check the volume setting before
each and every use.
Even though you may be thoroughly familiar with pipetting,
we suggest you practice the various pipetting methods we will
describe using water, a reservoir, and a 96 well plate.
To follow our directions for different pipetting procedures
you must first memorize the three trigger positions:
Conventional Method
A. Pull trigger to Position 2.
B. Insert tips several millimeters below the surface of the
liquid to be transferred.
C. Release trigger to Position 1. Remove from liquid, touching
tips to reservoir side to dispose of any excess liquid.
D. Dispense liquid by pulling trigger to position 2. Hesitate a
second or so and pull to Position 3 to blow out residue.
E. Change tips if necessary and repeat steps.
Alternate Method
This method is usually more accurate for dispensing very
small volumes of liquid. It may also be superior for liquids
that foam easily and for high viscosity fluids.
A. Pull trigger to Position 3.
B. Insert tips several millimeters below the surface of the
liquid to be transferred.
C. Release trigger to Position 1. Remove from liquid, touching
tips to reservoir side.
D. Dispense liquid by pulling trigger to Position 2. Touch tips
to liquid surface or well sides to release droplets.
E. Liquid remaining in tips may be returned to reservoir or
discarded.
Repeated Volume Dispensing Method
This is a quick and accurate method for multiple dispensing
of identical volumes.
A. Pull trigger to Position 3.
B. Insert tips several millimeters below the surface of the
liquid to be transferred.
C. Release trigger to Position 1. Remove from liquid, touching
tips to reservoir side.
D. Dispense liquid by pulling trigger to Position 2. Touch tips
to liquid surface or well sides to release droplets.
E. Return tips to reservoir and release trigger to Position 1.
F. Repeat steps D and E until pipetting is complete.
Figure 1. Head Settings Figure 2.Trigger Positions
100 µL 103.5 µL
Position 1
Trigger is released.
Position 2
Trigger pulled until stop
resistance is felt.
Position 3
Trigger pulled beyond stop,
as far it will go.
Manymanuals.com
Manymanuals.de
Manymanuals.fr
Manymanuals.it
Manymanuals.pl
Manymanuals.cz
Manymanuals.es
Manymanuals-pt.com
Comentarios a estos manuales